Triplex DNA.

Triplex DNA structures.

A DNA triplex is formed when pyrimidine or purine bases occupy the major groove of the DNA double Helix forming Hoogsteen pairs with purines of the Watson-Crick basepairs. Intermolecular triplexes are formed between triplex forming oligonucleotides (TFO) and target sequences on duplex DNA. Intramolecular triplexes are the major elements of H-DNAs, unusual DNA structures, which are formed in hom...

Triplex-Induced DNA Damage Response

Cellular DNA damage response is critical to preserving genomic integrity following exposure to genotoxic stress. A complex series of networks and signaling pathways become activated after DNA damage and trigger the appropriate cellular response, including cell cycle arrest, DNA repair, and apoptosis. The response elicited is dependent upon the type and extent of damage sustained, with the ultim...

Triplex DNA and human disease.

Mutagenesis is the fulcrum for the balance between the fidelity of the genetic code and evolution. While there are an enormous number of extrinsic factors driving mutagenesis, alternative DNA secondary structure is one of the intrinsic components that impacts regional genomic stability. Some alternative DNA structures are associated with human diseases, and this review focuses on disease-associ...

-stabilized self-assembly triplex DNA molecular switch

Trapping DNA polymerases using triplex-forming oligodeoxyribonucleotides.

Triplexes (triple helices) formed within DNA templates prior to or during DNA synthesis cause DNA polymerase to terminate [Samadashwily et al., EMBO J. 13 (1993) 4975-4983]. Here, we show that triplex-forming oligodeoxyribonucleotides (oligos) efficiently trap DNA polymerases at target DNA sequences within single-stranded (ss) templates. This was observed for all studied DNA polymerases, includ...